新聞中心
News Center
熱門搜索：
Fitzgerald Industries International代理
Envirologix 轉(zhuǎn)基因快速檢測試紙
Epitomics現(xiàn)貨抗體目錄
ABgene代理30
Cayman Chemical 特約代理
EVE™ Slide（EVE細(xì)胞計(jì)數(shù)板）
法國ID-VET
美國Sutter 玻璃毛坯 BF100-50-10
Sinobio公司產(chǎn)品介紹
Abcam代理
genview
D12079BWestern Diet(高純度飼料)
TEAA Solution（貨號：553303;SP5890）
LSPN-50ABMI公司LYSOSTAPHIN中國總代理
天然小鼠補(bǔ)體C1q蛋白
merck 2012年價(jià)格表
更新時(shí)間：2016-02-17
點(diǎn)擊次數(shù)：864
世界*實(shí)驗(yàn)材料供應(yīng)商 Worthington Biochemical 正式授權(quán)上海起發(fā)為其中國代理， Worthington Biochemical 在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 Worthington Biochemical 就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲
Worthington Biochemical 中國代理, Worthington Biochemical 上海代理， Worthington Biochemical 北京代理，Worthington Biochemical 廣東代理， Worthington Biochemical 江蘇代理Worthington Biochemical 湖北代理,Worthington Biochemical 天津,Worthington Biochemical 黑龍江代理,Worthington Biochemical 內(nèi)蒙古代理,Worthington Biochemical 吉林代理,Worthington Biochemical 福建代理, Worthington Biochemical 江蘇代理, Worthington Biochemical 浙江代理,Worthington Biochemical 四川代理,
Worthington組織消化指南
引言
組織消化酶主要用于組織培養(yǎng)和細(xì)胞生物學(xué)研究的原代細(xì)胞的分離和收集。盡管這些消化酶已經(jīng)普遍應(yīng)用多年，但是對其組織消化和細(xì)胞收集的作用機(jī)制卻沒有全面的理解。 由此導(dǎo)致的結(jié)果便是選擇一種消化方法大多是隨機(jī)選擇或基于過去的經(jīng)驗(yàn)，而不是在*理解消化原理的基礎(chǔ)上選擇的消化方案，以及如何根據(jù)具體實(shí)驗(yàn)?zāi)康男拚桨敢垣@得更好的實(shí)驗(yàn)結(jié)果。
細(xì)胞分離程序的目的是zui大程度的獲得功能活性的單個(gè)細(xì)胞，而影響結(jié)果的原因有很多，包括（不限于）以下：
1、組織類型
2、物種來源
3、動(dòng)物年齡
4、遺傳改造情況（如knockouts）
5、所用的消化培養(yǎng)液
6、所用的酶
7、消化酶中的雜質(zhì)
8、所用消化酶的工作濃度
9、溫度
10、孵育時(shí)間
通常前四個(gè)原因沒辦法選擇，而剩下的原因一般由經(jīng)驗(yàn)決定以獲取zui適實(shí)驗(yàn)結(jié)果。
通過查閱文獻(xiàn)來選擇*組織消化酶和zui適消化條件的研究人員通常被互相矛盾的實(shí)驗(yàn)數(shù)據(jù)搞得一頭霧水。這些巨大差異來源于組織胞外基質(zhì)的復(fù)雜而動(dòng)態(tài)的特性，以及過去常用的粗制、未提純的組織分離酶。另外，組織胞外基質(zhì)由各種蛋白、糖蛋白、脂類和糖脂組成，這些成分的組成比例在不同物種、不同組織、甚至不同發(fā)育（年齡）之間存在明顯的差異，這些因素都極大的影響了消化方案的制定。目前通常使用的粗制酶，如鏈霉蛋白酶、NF1:250、以及含有幾種其他不同濃度蛋白酶的膠原酶、以及各種多糖酶、核酶和脂酶。
本指南總結(jié)了目前常用組織消化酶的zui適消化和細(xì)胞收集知識和方法，描述了標(biāo)準(zhǔn)的實(shí)驗(yàn)步驟， 以便于制定一個(gè)*的細(xì)胞分離方案，同時(shí)列舉了一些相關(guān)的參考文獻(xiàn)。
下表列出多種組織的適宜消化酶（含參考文獻(xiàn)，以組織類型和物種分類，詳見鏈接）
脂肪組織 | 腎上腺組織 | 骨組織 | 腦組織 |
軟骨組織 | 結(jié)腸組織 | 內(nèi)皮組織 | 上皮組織 |
眼 | 心臟 | 腸 | 腎 |
肝 | 肺 | 淋巴結(jié) | 乳腺 |
其它組織 | 其他組織 | 肌組織 | 神經(jīng)組織 |
胰腺 | 腮腺 | 垂體 | 前列腺 |
生殖 | 動(dòng)物鱗片 | 皮膚 | 脾臟 |
干細(xì)胞 | 胸腺 | 甲狀腺/甲狀旁腺 | 扁桃體 |
腫瘤 |
|
|
|
注：使用Worthington公司消化酶的文獻(xiàn)不限于上表鏈接中所列，一般而言，Worthington公司的組織消化酶可用于大多組織類型的消化。
腎上腺組織分離手冊
| 物種 | 詳細(xì)來源 | 細(xì)胞名稱 | 消化用酶 | 培養(yǎng)基 | 參考文獻(xiàn) |
| 牛 | 牛 | Chromaffin | Collagenase Type 1: 0.1-0.2% | DMEM/F12 | Dominguez, N., Rodri璯uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012 |
| Deoxyribonuclease I: 0.003-0.015% | |||||
| Hyaluronidase: 0.015% | |||||
| 牛 | 牛, 6 個(gè)月 | Chromaffin | Collagenase Type 1: 0.125% | Locke's solution | Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of b綿羊 chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core, Cell Transplant 15, 121-33, 2006 |
| 牛 | 牛 | Chromaffin cells | Collagenase: 0.1% | DMEM | Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization., Mol Pharmacol 64, 1503, 2003 |
| Deoxyribonuclease I: 30 u/ml | |||||
| 牛 | 牛 | Chromaffin | Collagenase: 0.2% | Locke's solution | Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of B綿羊 Chromaffin Cell Xenografts in Rat CNS,Cell Transplant 1, 33, 1992 |
| 牛 | 牛 | Chromaffin | Deoxyribonuclease I: 30 u/mg | HEPES | Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in B綿羊 Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process, J Pharmacol Exp Ther 263 (3), 1479, 1992 |
| 牛 | 牛 | Heart | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B綿羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| (also 大鼠) | Adrenal chromaffin | ||||
| Paraneurons | |||||
| 牛 | 牛 | Chromaffin | Collagenase Type 1: 0.25 % | DMEM | Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells, J Neurochem 54 (3), 931, 1990 |
| 牛 | 牛 | Chromaffin | Collagenase: 0.2% | Locke's solution | Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on B綿羊 Chromaffin Cells, J Neurosci 7 (6), 1792, 1987 |
| Deoxyribonuclease I: 0.015% | |||||
| 牛 | 牛 | Chromaffin | Collagenase: 0.05% | Locke's solution, CMF | Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7, 89, 1985 |
| 牛 | 牛 | Chromaffin | Collagenase: 0.15% | Kreb's, CMF | Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells, Br J Biomed Sci 81, 599, 1984 |
| 牛 | 牛 | Chromaffin | Collagenase: 0.1% | (see reference) | Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.:An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells, J Biol Chem 259, 1114, 1984 |
| 牛 | 牛 | Chromaffin | Collagenase Type 1: 0.05% | CF Kreb's | Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells, Br J Pharmacol 81, 119, 1984 |
| 牛 | 牛 | Chromaffin | Collagenase Type 1: 0.025% | HBSS, modified | Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: B綿羊 Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture, J Neurosci Methods 7, 329, 1983 |
| 牛 | 牛 | Medulla | Hyaluronidase: 0.2% | Saline w/BSA 0.5% | Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated B綿羊 Adrenal Medullary Cells, Q J Exp Physiol 68, 123, 1983 |
| 牛 | 牛 | Medulla | Collagenase: 0.2% | Krebs-Ringer bicarbonate buffer, CMF | Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells, Cell Tissue Res226, 655, 1982 |
| 牛 | 牛 | Chromaffin | Deoxyribonuclease I: 15 礸/ml | Medium A | Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium, Exp Cell Res 133, 159, 1981 |
| (see reference) | |||||
| 牛 | 牛 | Medulla | Protease: 0.2% | Saline | Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in B綿羊 Adrenal Medullary Cells, Phil Trans R Soc Lond 296, 83, 1981 |
| 牛 | 牛 | Chromaffin | Collagenase: 0.05% | Locke's solution, CMF | Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in B綿羊 Adrenal Chromaffin Cell Cultures, Neuroscience 5, 1533, 1980 |
| 牛 | 牛, 成年的 | Chromaffin | Collagenase: 0.5% | HBSS | Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980 |
| (還有大鼠, 加拿大-威斯塔, 年幼的; 豚鼠, 初生) | |||||
| 牛 | 牛 | Chromaffin | Collagenase: 0.25% | F-12 medium | Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells, Nature 283, 489, 1980 |
| 牛 | 牛 | Medulla | Collagenase: 0.05% | Locke's solution, CF | Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of B綿羊 Adrenal Medulla Cells in Culture, J Neurochem 35 (3), 679, 1980 |
| 牛 | 牛, 成年的 | Medulla | Collagenase Type 1: 0.5% | HBSS | Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated B綿羊 Adrenal Medullary Cells in Long-Term Cultures,Neuroscience 5, 1445, 1980 |
| 牛 | 牛 | Chromaffin | Collagenase: 0.05% | DMEM | Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.:Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells, Neuroscience 5, 2261, 1980 |
| 牛 | 牛 | Medullary | Collagenase Type 2: 0.2% | HEPES | Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured B綿羊 Adrenal Medullary Cells, J Pharmacol Exp Ther 209 (1), 147, 1979 |
| 牛 | 牛 | Foreskin | Collagenase: 0.5% | Dulbecco's MEM w/10% calf serum | Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979 |
| 牛 | 牛 | Medulla | Collagenase Type 1: 0.05% | Kreb's, CF | Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated B綿羊 Adrenal Medullary Cell, J Cell Biol 76, 12, 1978 |
| 牛 | 牛 | Chromaffin | Hyaluronidase: 0.2% | HEPES, CF | Brooks, J.C.: The Isolated B綿羊 Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion, Endocrinology 101, 1369, 1977 |
| 牛 | 牛, 雄性 | Chromaffin | Collagenase Type 1: 0.2% | Kreb's Ringer bicarbonate buffer, CF | Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla, Proc Natl Acad Sci U S A 74, 5036, 1977 |
| 豚鼠 | 豚鼠, 500-700克 | Chromaffin | Collagenase: | BSS | Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of 豚鼠 Adrenal Medulla, Neurochem Int 6, 1813, 1981 |
| Medulla | (see reference) | ||||
| 豚鼠 | 豚鼠 | Chromaffin | Collagenase: 0.5% | HBSS | Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980 |
| (還有大鼠,漢諾威-威斯塔, 年幼的; 初生; 牛) | |||||
| 豚鼠 | 豚鼠 | Adrenal | Collagenase: 0.05%-0.20% | Kreb's-Ringer bicarb glucose buffer, CF | Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells, Biochim Biophys Acta 421, 168, 1976 |
| Chromaffin | |||||
| 倉鼠 | 倉鼠 (金倉鼠) 100-150 克 | Adrenal | Hyaluronidase: 0.20% | Kreb's Ringer bicarbonate buffer | Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by 倉鼠 Adrenal Cells, J Neurochem 32, 927, 1979 |
| Chromaffin | |||||
| 人類 | 人類 | Adrenal medulla progenitor | Collagenase: 0.2% | DMEM/F-12 | Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from 人類 Adult Adrenal Medulla., Stem Cells Transl Med 1, 783, 2012 |
| 人類 | 人類 | Chromaffin cells | Collagenase: 0.2% | Locke's solution | Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured 人類 Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model., Korean J Anesthesiol 60, 357, 2011 |
| 人類 | 人類 | Adrenocortical | Collagenase Type 1: 0.2% | Krebs Ringer | Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of 人類 Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method., Endocrinology 151, 1375, 2010 |
| Deoxyribonuclease I: 0.01% | |||||
| 人類 | 人類, 成年的 | Chromaffin | Trypsin: 0.25% | Eagle's MEM | Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal 人類 Chromaffin Cells, Lab Invest 43, 399, 1980 |
| 人類 | 人類, 成年的和小孩 | Foreskin | Collagenase: 0.5% | Dulbecco's MEM w/10% calf serum | Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979 |
| 小鼠 | 小鼠 | Chromaffin | Collagenase Type 1: 0.1-0.2% | DMEM/F12 | Dominguez, N., Rodri璯uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012 |
| Deoxyribonuclease I: 0.003-0.015% | |||||
| Hyaluronidase: 0.015% | |||||
| 小鼠 | 小鼠, 8-10 周 | Chromaffin | Papain: 40 u/ml | DMEM | Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: 小鼠 Adrenal Chromaffin Cell Isolation., J Vis Exp 2, 129, 2007 |
| 小鼠 | 小鼠, 胚胎 | Chromafin cells | Papain: 20-25 u/ml | DMEM | Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells, J Neurosci 25, 10546-55, 2005 |
| 綿羊 | 綿羊, 成年的和胚胎 | Adrenocortical | Collagenase Type 1: 0.4% | DMEM/Ham's F12 | Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in 綿羊 Adrenocortical Cells., Reprod Sci 17, 477, 2010 |
| 綿羊 | 綿羊, 胚胎 | Adrenocortical | Collagenase Type 1: 0.4% | DMEM/Ham's F12 | Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.:Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression.,Am J Physiol Regul Integr Comp Physiol 289, R410, 2005 |
| 綿羊 | 綿羊, 成年 | Chromaffin cells | Collagenase Type 2: 0.2% | Locke's solution | Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels., J Physiol555, 489, 2004 |
| Deoxyribonuclease I: 100 u/ml | |||||
| 綿羊 | 綿羊, 3 年 | Anterior pituitary | Trypsin: 2.5% | DMEM | Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep, J Endocrinol 162, 215-25, 1999 |
| Deoxyribonuclease I: 0.004% | |||||
| 大鼠 | 大鼠 | Chromaffin | Collagenase Type 1: 0.1-0.2% | DMEM/F12 | Dominguez, N., Rodri璯uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012 |
| Deoxyribonuclease I: 0.003-0.015% | |||||
| Hyaluronidase: 0.015% | |||||
| 大鼠 | 大鼠 | Chromaffin | Collagenase Type 1: 0.26% | HBSS | Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays,Nat Protoc , 294, 2006 |
| Deoxyribonuclease I: 0.015% | |||||
| Hyaluronidase: 0.015% | |||||
| 大鼠 | 大鼠, SD | Chromaffin cells | Collagenase Type 1: 0.26% | HBSS | Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L., Instituto Teofilo Hernando, , 2004 |
| Deoxyribonuclease I: 0.015% | |||||
| Hyaluronidase: 0.015% | |||||
| 大鼠 | 大鼠, SD, 雄性 | Zona fasciculata/reticularis | Collagenase: 0.4% | Krebs-HEPES | Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells, Am J Physiol Regul Integr Comp Physiol 284, R1631-5, 2003 |
| 大鼠 | 大鼠, 威斯塔, 初生 | Chromaffin cells | Collagenase Type 1: 0.025% | DMEM | Zhang L, Casl A, Avila E, Drucker-Col鉚n R, Escobar A:Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons, J Neuropathol Exp Neurol 59, 170-4, 2000 |
| Deoxyribonuclease I: 0.0015% | |||||
| 大鼠 | 大鼠, SD, 雄性 | ZG | Collagenase Type 1: 0.2% | Kreb's | Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis, J Endocrinol 154, 449, 1997 |
| ZFR | |||||
| 大鼠 | 大鼠, 費(fèi)希爾, 雄性, 10-16周 | Adrenocortical | Deoxyribonuclease I: 0.005% | BSS | Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture,In Vitro Cell Dev Biol 26, 493, 1990 |
| 大鼠 | 大鼠, 雄性, 120-160 g, | Leydig | Collagenase Type 2: 0.03% (adrenal) | Krebs Ringer bicarbonate buffer | Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26, 24, 1990 |
| 大鼠, SD, 雄性, 400-450 g | Adrenal | ||||
| 大鼠 | 大鼠, SD, 2-4 day old | Heart | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and B綿羊 Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| (also 牛) | Adrenal chromaffin | ||||
| Paraneurons | |||||
| 大鼠 | 大鼠, Long-Evans, 雌性, 150-200 g | Glomerulosa | Collagenase: 0.2% | MEM-d-Val | Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture, J Steroid Biochem 20, 449, 1984 |
| 大鼠 | 大鼠, 費(fèi)希爾, 雄性, 1-10月 | Adrenocortical | Deoxyribonuclease I: 0.005% | BSS | Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells, In Vitro 19, 46, 1983 |
| 大鼠 | 大鼠, SD, 雄性, 400-450 克 | Decapular | Deoxyribonuclease I: 0.01% | Medium 199 | Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells, Int J Pept Protein Res 19, 361, 1982 |
| Capsular | |||||
| Glomerulosa | |||||
| 大鼠 | 大鼠 | Chromaffin | Trypsin: 0.10% | Ham's F-12 w/HEPES | Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells, Exp Cell Res 125, 369, 1980 |
| 大鼠 | 大鼠, 漢諾威-威斯塔, 第二出生后的一周 (還有 豚鼠, 牛) | Chromaffin | Collagenase: 0.5% | HBSS | Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells, Adv Biochem Psychopharmacol 25, 51, 1980 |
| 大鼠 | 大鼠 , SD, 雌性, 200 克 | Glomerulosa | Deoxyribonuclease I: 0.05% | Kreb's | Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells, Endocrinology 106 (1), 50, 1980 |
| 大鼠 | 大鼠 | Foreskin | Collagenase: 0.5% | Dulbecco's MEM w/10% calf serum | Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979 |
| 大鼠 | 大鼠, 漢諾威-威斯塔, 7-12 天 | Medullary | Trypsin: 0.125% | HBSS | Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids, Proc Natl Acad Sci U S A 75 (7), 3498, 1978 |
| 大鼠 | 大鼠, SD, 雄性 | Cortical | Trypsin: 0.25% | Kreb's Ringer bicarbonate buffer | Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands,Exp Cell Res 79, 263, 1973 |
| 大鼠 | 大鼠, Holtzman, 雄性, 180-250 g | Adrenal | Collagenase Type 1: 0.5% | Kreb's Ringer bicarbonate buffer | Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism, Endocrinology 82, 1053, 1968 |
我們公司zui大優(yōu)勢是強(qiáng)大的采購，
1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，
2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，
3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免
4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證100%產(chǎn)品都是,因?yàn)橐馕吨鴽]有服務(wù).
5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大 交大 復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥 ，fisher等500多家公司
6：我們還是Santa,Advanced Biotechnologies Inc, Worthington Biochemical ,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. Dako,Inc, GePromegaridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,Worthington Biochemical , Polysciences,prospecbi, QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。
7：我們還是invitrogen,qiagen,Worthington Biochemical am,sigma;Promega,roche,merck, rnd,BD, GE,pierce,BioLegend等*批發(fā)，歡迎合作。
當(dāng)前位置：
